Day 1: The first day covers a set of microscopy methods to measure molecular dynamics, i.e. the mobility, stability, interactions and conformational changes of molecules or particles. An introduction to Fluorescence Recovery After Photobleaching (FRAP), Single Particle Tracking (SPT), single molecule Fluorescence Resonance Energy Transfer (smFRET), Fluorescence Correlation Spectroscopy (FCS) and Raster Imaging Correlation Spectroscopy (RICS) will be given followed by demonstration sessions of each technique/experimental set-up.

UGent, campus Heymans

07-10-2022 09:00 - 18:00

Day 2: During the second day we will focus on mesoscale imaging and non-linear optical (NLO) imaging. Mesoscale imaging is a general term referring to various imaging methods that allow the visualization of larger (up to cm) samples while maintaining subcellular resolution. Two specific techniques will be demonstrated: light sheet microscopy and optical projection tomography. Non-linear imaging involves the visualization of (label-free) contrast mechanisms in which at least 2 photons are recombined into a single photon. Due to inherent optical sectioning, greater optical range and the less invasive nature of red-shifted photons employed in NLO imaging this enables live, three dimensional imaging of larger volumes over extended periods of time.

UA

18-11-2022 09:00 - 18:00

Day 3: On the third day, we will zoom in on super-resolution microscopy. Super-resolution microscopy refers to a collection of techniques that are able to circumvent the fundamental resolution limit imposed by diffraction through various means. The techniques covered include: Airyscan, SIM (SIM-TIRF), SML and STED. Lectures will explain the theory behind the different principles to break the diffraction barrier employed in each technique. Practical sessions will illustrate the possibilities, advantages and disadvantages of enhanced resolution imaging.

KUL

08-12-2022 09:00 - 18:00